Establishment and Transformation of Callus and Cell Suspension Cultures of the Prickly-Pear (Opuntia ficus-indica)

Authors

  • R. M. Llamoca-Zárate
  • F. A. P. Campos
  • J. Landsmann

Keywords:

Cell suspension, friable callus, Opuntia ficus indica, particle gun

Abstract

Friable callus cultures were initiated from cotyledons and hypocotils of Opuntia ficus-indica.
Propagation and growth of calli were observed on MS medium supplemented with 2,4-D, Pickloram
and Casein. Cell transformation was achieved by particle bombardment. Kanamycin resistance,
GUS expression, and PCR analyses indicated successful integration of foreign DNA.

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Published

18-04-1998 — Updated on 20-06-2020

Issue

Section

Scientific Papers