Establishment and Transformation of Callus and Cell Suspension Cultures of the Prickly-Pear (Opuntia ficus-indica)

R. M. Llamoca-Zárate; F. A. P. Campos; J. Landsmann

doi:10.56890/jpacd.v3i.140

Authors

R. M. Llamoca-Zárate
F. A. P. Campos
J. Landsmann

DOI:

https://doi.org/10.56890/jpacd.v3i.140

Keywords:

Cell suspension, friable callus, Opuntia ficus indica, particle gun

Abstract

Friable callus cultures were initiated from cotyledons and hypocotils of Opuntia ficus-indica.
Propagation and growth of calli were observed on MS medium supplemented with 2,4-D, Pickloram
and Casein. Cell transformation was achieved by particle bombardment. Kanamycin resistance,
GUS expression, and PCR analyses indicated successful integration of foreign DNA.