Effect of substrate and gibberellic acid (GA3) on seed germination in ten cultivars of Opuntia sps.

Authors

  • María Judith Ochoa
  • Luz Maritza González-Flores
  • José Manuel Cruz-Rubio
  • Liberato Portillo
  • Juan Florencio Gómez-Leyva

DOI:

https://doi.org/10.56890/jpacd.v17i.61

Keywords:

cactus pear fruit, scarification, substrate, in vitro germination, ?-amylase

Abstract

The seeds of Opuntia sps show low germination capacity and high latency, so this study
compares different inert and synthetic substrates in vitro with the addition of gibberellic acid
(GA3) to stimulate seed germination in ten cultivars of Opuntia sps. (O. megacantha, O.
amyclaea, O. streptacantha, O. albicarpa and O. ficus-indica). The photoperiod 16/8 h,
light/darkness had a positive stimulating effect on the seed germination rate, while an inert
substrate (pumice:peat moss:sand, 1:1:1 v/v) showed on average 20% germination. The use
of in vitro Gamborg medium increased the germination rate to 40%. The addition of 25 mg L-1
gibberellic acid (GA3) to Murashige and Skoog (1962) medium, prepared to ¼ basal salt
concentration, increases the germination rate across all cultivars, up to 90% at four weeks.
GA3 was shown to play an important role in breaking the latency of the seed, and increased
amylolytic activity during seed germination in Opuntia sps.

Publication Facts

Metric
This article
Other articles
Peer reviewers 
0
2.4

Reviewer profiles  N/A

Author statements

Author statements
This article
Other articles
Data availability 
N/A
16%
External funding 
N/A
32%
Competing interests 
N/A
11%
Metric
This journal
Other journals
Articles accepted 
17%
33%
Days to publication 
1874
145

Indexed in

Editor & editorial board
profiles
Publisher 
Professional Association for Cactus Development

Downloads

Published

24/09/2024

Issue

Section

Scientific Papers